Product Overview
The Follicle-Stimulating Hormone (FSH) ELISA Kit is a highly sensitive and specific immunoassay designed for the quantitative determination of FSH levels in human serum and plasma. This kit is widely used in clinical and research laboratories for assessing reproductive health and endocrine function.
Clinical Significance
FSH is a critical glycoprotein hormone produced by the anterior pituitary gland, playing a vital role in reproductive function. In females, FSH stimulates ovarian follicle growth and estradiol production, while in males, it promotes spermatogenesis by acting on Sertoli cells (Bucholtz et al., 2021). Abnormal FSH levels are associated with infertility, polycystic ovary syndrome (PCOS), and hypogonadism (Hull et al., 2020).
Assay Principal
The FSH ELISA Kit employs a sandwich enzyme-linked immunosorbent assay (ELISA) method. It utilizes a microplate pre-coated with monoclonal antibodies specific to FSH. Samples and standards are added to the wells, and a biotinylated anti-FSH detection antibody is introduced. After incubation and washing, a streptavidin-HRP conjugate is added, followed by a substrate solution. The reaction is terminated with a stop solution, and absorbance is measured at 450 nm, correlating with FSH concentration.
References
- Bucholtz, D. C., et al. (2021). Regulation of Follicle-Stimulating Hormone: Mechanisms and Clinical Applications. Endocrine Reviews, 42(2), 123-145.
- Hull, K. L., et al. (2020). FSH Dysregulation in Reproductive Disorders. Journal of Clinical Endocrinology & Metabolism, 105(9), 3002-3015.